All products Antibodies HA-probe Antibody (Y-11) PCPC5

HA-probe Antibody (Y-11) PCPC5

Catalog Number:OM156743

  • Western blot analysis of HA-probe expression in HA tagged β2B Tubulin (A), HA tagged 3pk (B) and HA tagged PTP 1B (C) transfected lysates.

  • Antibodies tested include HA-probe (Y-11)-G: (A) and HA-probe (Y-11): (B,C).

  • Immunofluorescence staining of methanol-fixed COS cells transfected with HA fusion protein showing cytoplasmic staining.

Product Profile

Product Name HA-probe Antibody (Y-11) PCPC5
Antibody Type Primary Antibodies
Modification Notes Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. For example, the pCDM8 expression vector and derivatives thereof encode fusions between the target protein and an 11 amino acid peptide derived from the influenza protein hemagglutinin (HA). The HA epitope tag is useful in Western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-epitope tag.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFFCELISAChIP

recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope mapping within an internal region of the influenza hemagglutinin (HA) protein
Tissue Specificity epitope mapping within an internal region of the influenza hemagglutinin (HA) protein

Application

  • Application

    Western blot analysis of HA-probe expression in HA tagged β2B Tubulin (A), HA tagged 3pk (B) and HA tagged PTP 1B (C) transfected lysates.

  • Application

    Antibodies tested include HA-probe (Y-11)-G: (A) and HA-probe (Y-11): (B,C).

  • Application

    Immunofluorescence staining of methanol-fixed COS cells transfected with HA fusion protein showing cytoplasmic staining.

Application Notes
recommended for detection of proteins containing the HA tag by WB, IP, IF, FCM and ELISA:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM156743
  • Price: $336.00
  • Discount Price
  • Size:
  • Store: Out of stock
  • Lead time: 4~6 Week
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