MINA Antibody: MINA is nuclear localized, myc-inducible protein that is thought to play a role in mammalian cell proliferation. Treatment of cancer cells lines such as the colon cancer cell line SW680 with siRNA against MINA inhibits cell growth, demonstrating that MINA may be a potential therapeutic target. MINA regulates several genes related to cell adhesion and metabolism that have also been shown to be regulated by c-Myc, but also regulates other genes whose expression are not modulated by c-Myc such as EGFR, IL-6 and HGF. MINA has also been found to act as a repressor to IL-4 expression in T cells, indicating that it may also play a role in T cell differentiation and genetic variation in T helper type 2 bias.1) Tsuneoka M, Kody Y, Soejima M, et al. A novel myc target gene, mina53, that is involved in cell proliferation. J. Biol. Chem.2002; 277:35450-9.
Immunogen
MINA antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human MINA. The immunogen is located within amino acids 380 - 430 of MINA.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAIFIHC-PWB
MINA antibody can be used for detection of MINA by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:
MINA Antibody: ROX MDIG NO52 MINA53 Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA 60S ribosomal protein L27a histidine hydroxylase ROX
Sequence Similarities
Predicted species reactivity based on immunogen sequence: Bovine: (80%)
Immunohistochemistry of MINA in mouse heart tissue with MINA antibody at 5 μg/mL.
Application
Western blot analysis of MINA in human heart tissue lysate with MINA antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of MINA in mouse heart tissue with MINA antibody at 20 μg/mL.
Positive Control
1) Cat. No. 1301 - Human Heart Tissue Lysate
Application Notes
MINA antibody can be used for detection of MINA by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.:
Additional Information
Form
Liquid
Storage Instructions
MINA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
MINA Antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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