Western blot analysis of MYC using rabbit polyclonal MYC Antibody (S373) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MYC gene (Lane 2).
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.
MYC is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of the gene encoding MYC have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.1) Lima,F.P., Am. J. Clin. Pathol. 129 (5), 723-726 (2008)
Immunogen
This MYC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-380 amino acids from human MYC.
Western blot analysis of MYC using rabbit polyclonal MYC Antibody (S373) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MYC gene (Lane 2).
Application
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
Search the catalog number from any major antibody supplier/brand in the search bar above. The search engine will return OmnimAbs products for these targets.