All products Antibodies NDUFS4 Antibody (C-term)

NDUFS4 Antibody (C-term)

Catalog Number：OM215145

  • Western blot analysis of NDUFS4 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). NDUFS4 (arrow) was detected using the purified Pab.

  • Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with NDUFS4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Flow cytometric analysis of ATDC5 cells using SLC22A23 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Western blot analysis of NDUFS4 (arrow) using rabbit polyclonal NDUFS4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDUFS4 gene.

Product Profile

Product Name NDUFS4 Antibody (C-term)
Antibody Type Primary Antibodies

Key Feature

Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications

WBIHCFC
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:
Species Reactivity

Human
Concentration 1 mg/ml
Purification

Target Information

Alternative Names
NDUFS4
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4
mitochondrial
Complex I-18 kDa
Complex I-AQDQ
NADH-ubiquinone oxidoreductase 18 kDa subunit
Molecular Weight(MW) 20108 Da
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
Tissue Specificity This NDUFS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138~168 amino acids from the C-terminal region of human NDUFS4.
Cellular Localization Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Database Links

Entrez Gene 4724

Application

  • Application

    Western blot analysis of NDUFS4 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). NDUFS4 (arrow) was detected using the purified Pab.

  • Application

    Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with NDUFS4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

  • Application

    Flow cytometric analysis of ATDC5 cells using SLC22A23 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Application

    Western blot analysis of NDUFS4 (arrow) using rabbit polyclonal NDUFS4 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDUFS4 gene.

Application Notes
WB~~1:100~500 IHC~~1:50~100 FC~~1:10~50:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM215145
  • Price: $336.00
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