Rabbit Anti-Chicken IgY (H&L) Antibody (Alkaline Phosphatase)

Product Profile

Product Name	Rabbit Anti-Chicken IgY (H&L) Antibody (Alkaline Phosphatase)
Antibody Type	Secondary Antibodies
Immunogen	Chicken IgY - whole molecule

Key Feature

Clonality	Polyclonal
Isotype	IgY
Host Species	Rabbit
Tested Applications	ELISA
	Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user).:
Species Reactivity	Chicken
Concentration	1 mg/ml
Purification	Affinity purified

Target Information

Target Description	Reacts with the heavy chain of chicken IgY.

Application

Application Notes

Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user).:

Additional Information

Form	Liquid
Storage Instructions	Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY can be stored vial at 4 ˚C before opening. DO NOT FREEZE. This product is stable at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Storage Buffer	0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.1% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (BSA)

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• Data sheet for OM296266