Sample Type: human colon tissues infected ex-vivo with HIV-1
Green: Primary
Blue: DAPI
Primary Dilution: 1:100
Secondary Antibody: Donkey anti-Rabbit AF 488
Secondary Dilution: 1:500
Image Submitted By: Chiara Foglieni
San Raffaele Scientific Institute, Milan, Italy
Immunohistochemistry with HMEC-1 and A549 cells tissue
Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extract
Primary Antibody Dilution : 1:500
Secondary Antibody : Anti rabbit-HRP
Secondry Antibody Dilution : 1:5,000
Submitted by: Scott Wilson, University of Alabama at Birmingham
CXCR4 antibody - N-terminal region validated by WB using 721_B cell Lysate at 0.2-1 ug/ml.
CXCR4 antibody - N-terminal region validated by WB using human microvascular endothelial cells (25ug) at 1:1000.
0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate
Product Name | Rabbit anti-CXCR4 polyclonal antibody - N-terminal region |
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Antibody Type | Primary Antibodies |
Immunogen | The immunogen for anti-CXCR4 antibody: synthetic peptide directed towards the N terminal of human CXCR4 |
Clonality | Polyclonal |
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Isotype | IgG |
Host Species | Rabbit |
Tested Applications | |
FC:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
Species Reactivity | |
Concentration | 1 mg/ml |
Purification | Affinity purified |
Gene Symbol | CXCR4 |
---|---|
Gene Synonyms | CD184 D2S201E FB22 HM89 HSY3RR LAP3 LCR1 LESTR NPY3R NPYR NPYRL NPYY3R WHIM |
Gene Full Name | Chemokine (C-X-C motif) receptor 4 |
Gene Summary | CXCR4 is a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
Alternative Names | CD184 D2S201E FB22 HM89 HSY3RR LAP3 LCR1 LESTR NPY3R NPYR NPYRL NPYY3R WHIM |
Molecular Weight(MW) | 40kDa |
Sequence | 352 amino acids |
Entrez Gene | 7852 |
---|---|
SwissProt ID | P61073 |
Protein Accession | NP_003458 |
Sample Type: human colon tissues infected ex-vivo with HIV-1
Green: Primary
Blue: DAPI
Primary Dilution: 1:100
Secondary Antibody: Donkey anti-Rabbit AF 488
Secondary Dilution: 1:500
Image Submitted By: Chiara Foglieni
San Raffaele Scientific Institute, Milan, Italy
Immunohistochemistry with HMEC-1 and A549 cells tissue
Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extract
Primary Antibody Dilution : 1:500
Secondary Antibody : Anti rabbit-HRP
Secondry Antibody Dilution : 1:5,000
Submitted by: Scott Wilson, University of Alabama at Birmingham
CXCR4 antibody - N-terminal region validated by WB using 721_B cell Lysate at 0.2-1 ug/ml.
CXCR4 antibody - N-terminal region validated by WB using human microvascular endothelial cells (25ug) at 1:1000.
0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 721_B cell lysate
Application Notes | FC:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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Form | Liquid |
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Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
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