All products Antibodies Rabbit anti-CXCR4 polyclonal antibody - N-terminal region

Rabbit anti-CXCR4 polyclonal antibody - N-terminal region

Catalog Number:OM105220

  • Sample Type: human colon tissues infected ex-vivo with HIV-1
    Green: Primary
    Blue: DAPI
    Primary Dilution: 1:100
    Secondary Antibody: Donkey anti-Rabbit AF 488
    Secondary Dilution: 1:500
    Image Submitted By: Chiara Foglieni
    San Raffaele Scientific Institute, Milan, Italy 

  • Immunohistochemistry with HMEC-1 and A549 cells tissue

  • Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extract
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Anti rabbit-HRP
    Secondry Antibody Dilution : 1:5,000
    Submitted by: Scott Wilson, University of Alabama at Birmingham

  • CXCR4 antibody - N-terminal region validated by WB using 721_B cell Lysate at 0.2-1 ug/ml.

  • CXCR4 antibody - N-terminal region validated by WB using human microvascular endothelial cells (25ug) at 1:1000.

  • 0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: 721_B cell lysate

Product Profile

Product Name Rabbit anti-CXCR4 polyclonal antibody - N-terminal region
Antibody Type Primary Antibodies
Immunogen
The immunogen for anti-CXCR4 antibody: synthetic peptide directed towards the N terminal of human CXCR4

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

FCIHCWB

FC:1:200~1:500
IHC:1:50~1:200
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

HumanRabbit

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol CXCR4
Gene Synonyms
CD184
D2S201E
FB22
HM89
HSY3RR
LAP3
LCR1
LESTR
NPY3R
NPYR
NPYRL
NPYY3R
WHIM
Gene Full Name Chemokine (C-X-C motif) receptor 4
Gene Summary
CXCR4 is a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Alternative Names
CD184
D2S201E
FB22
HM89
HSY3RR
LAP3
LCR1
LESTR
NPY3R
NPYR
NPYRL
NPYY3R
WHIM
Molecular Weight(MW) 40kDa
Sequence 352 amino acids

Database Links

Entrez Gene 7852
SwissProt ID P61073
Protein Accession NP_003458

Application

  • Immunohistochemical

    Sample Type: human colon tissues infected ex-vivo with HIV-1
    Green: Primary
    Blue: DAPI
    Primary Dilution: 1:100
    Secondary Antibody: Donkey anti-Rabbit AF 488
    Secondary Dilution: 1:500
    Image Submitted By: Chiara Foglieni
    San Raffaele Scientific Institute, Milan, Italy 

  • Immunohistochemistry

    Immunohistochemistry with HMEC-1 and A549 cells tissue

  • Western blot

    Positive Control: Lane 1: 20ug mouse brain extract Lane 2: 20ug mouse brain extract
    Primary Antibody Dilution : 1:500
    Secondary Antibody : Anti rabbit-HRP
    Secondry Antibody Dilution : 1:5,000
    Submitted by: Scott Wilson, University of Alabama at Birmingham

  • Western blot

    CXCR4 antibody - N-terminal region validated by WB using 721_B cell Lysate at 0.2-1 ug/ml.

  • Western blot

    CXCR4 antibody - N-terminal region validated by WB using human microvascular endothelial cells (25ug) at 1:1000.

  • Western blot

    0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: 721_B cell lysate

Application Notes
FC:1:200~1:500
IHC:1:50~1:200
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM105220
  • Price: $336.00
  • Discount Price
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  • Store: In stock
  • Lead time: 2~3 Week
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