All products Antibodies Rabbit anti-HSPA9 polyclonal antibody - C-terminal region

Rabbit anti-HSPA9 polyclonal antibody - C-terminal region

Catalog Number：OM105141

  • 500 ug mouse brain homogenate
    Amount of IP Antibody :
    6 ug
    Primary Antibody :
    HSPA9
    Primary Antibody Dilution :
    1:500
    Secondary Antibody :
    Goat anti-rabbit Alexa-Fluor 594
    Secondary Antibody Dilution :
    1:5000
    Gene Name :
    HSPA9
    Submitted by :
    Dr. Yuzhi Chen, University of Arkansas for Medical Science

  • Human brain stem cells
    Primary Antibody Dilution :
    1:500
    Secondary Antibody :
    Goat anti-rabbit Alexa-Fluor 594
    Secondary Antibody Dilution :
    1:1000
    Color/Signal Descriptions :
    HSPA9: Red DAPI:Blue
    Gene Name :
    HSPA9
    Submitted by :
    Dr. Yuzhi Chen, University of Arkansas for Medical Science

  • Skeletal muscle

  • Data Provided by Dr. Yuzhi Chen, University of Arkansas for Medical SciencesNT2 cells were pretreated with 2N HCL for 30 minutesWashed 3x in PBSStained for 2 hours with 1ug/50ul antibodyand Incubated in Alexa goat-rabbit 594 for 1H.Images were obtained with a 40x objectiveNT-2 cellsAntibody: RedDAPI: Blue

  • 0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: 293T cell lysate

Product Profile

Product Name Rabbit anti-HSPA9 polyclonal antibody - C-terminal region
Antibody Type Primary Antibodies
Immunogen 
The immunogen for anti-HSPA9 antibody: synthetic peptide directed towards the C terminal of human HSPA9

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIHCIP
WB:1:500~1:2000
IHC:1:50~1:200
IP:1:100~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

DogHorseHumanMousePigRabbitRat
Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol HSPA9
Gene Synonyms
CSA
GRP75
HSPA9B
MGC4500
MOT
MOT2
MTHSP75
PBP74
mot-2
GRP-75
Gene Full Name Heat shock 70kDa protein 9 (mortalin)
Gene Summary
HSPA9 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA9 is a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone.CSHL1 is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. This particular family member is expressed in placental villi, although it was originally thought to be a pseudogene. In fact, alternative splicing suggests that the majority of the transcripts would be unable to express a secreted protein.The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants have been observed, but most of them are not thought to be protein-coding.
Alternative Names
CSA
GRP75
HSPA9B
MGC4500
MOT
MOT2
MTHSP75
PBP74
mot-2
GRP-75
Molecular Weight(MW) 75kDa
Sequence 679 amino acids

Database Links

Entrez Gene 3313
SwissProt ID P38646
Protein Accession NP_004125

Application

  • Western blot

    500 ug mouse brain homogenate
    Amount of IP Antibody :
    6 ug
    Primary Antibody :
    HSPA9
    Primary Antibody Dilution :
    1:500
    Secondary Antibody :
    Goat anti-rabbit Alexa-Fluor 594
    Secondary Antibody Dilution :
    1:5000
    Gene Name :
    HSPA9
    Submitted by :
    Dr. Yuzhi Chen, University of Arkansas for Medical Science

  • Immunofluorescence

    Human brain stem cells
    Primary Antibody Dilution :
    1:500
    Secondary Antibody :
    Goat anti-rabbit Alexa-Fluor 594
    Secondary Antibody Dilution :
    1:1000
    Color/Signal Descriptions :
    HSPA9: Red DAPI:Blue
    Gene Name :
    HSPA9
    Submitted by :
    Dr. Yuzhi Chen, University of Arkansas for Medical Science

  • Immunohistochemical

    Skeletal muscle

  • Immunohistochemical

    Data Provided by Dr. Yuzhi Chen, University of Arkansas for Medical SciencesNT2 cells were pretreated with 2N HCL for 30 minutesWashed 3x in PBSStained for 2 hours with 1ug/50ul antibodyand Incubated in Alexa goat-rabbit 594 for 1H.Images were obtained with a 40x objectiveNT-2 cellsAntibody: RedDAPI: Blue

  • Western blot

    0.2-1 ug/ml
    ELISA Titer: 1:62500
    Positive Control: 293T cell lysate

Application Notes
WB:1:500~1:2000
IHC:1:50~1:200
IP:1:100~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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  • Catalog Number: OM105141
  • Price: $336.00
  • Discount Price
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  • Store: In stock
  • Lead time: 2~3 Week

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