All products Antibodies Rabbit anti-IKBKB polyclonal antibody - middle region

Rabbit anti-IKBKB polyclonal antibody - middle region

Catalog Number:OM105584

  • Lane 1: 10ug 293T lysate (empty vector)
    Lane 2: 10ug IKKalpha-V5 transfected 293T lysate
    Lane 3: 10ug IKKbeta-V5 transfected 293T
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Anti-rabbit HRP
    Secondary Antibody Dilution:
    1:2000
    Gene Name:
    IKBKB
    Submitted by:
    Dr. Tencho Tenev, The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research

  • 0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: Human brain

Product Profile

Product Name Rabbit anti-IKBKB polyclonal antibody - middle region
Antibody Type Primary Antibodies
Immunogen
The immunogen for anti-IKBKB antibody: synthetic peptide directed towards the middle region of human IKBKB

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB

WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

BovineDogHorseHumanMousePigRat

Concentration 1 mg/ml
Purification Affinity purified

Target Information

Gene Symbol IKBKB
Gene Synonyms
FLJ40509
IKK-beta
IKK2
IKKB
MGC131801
NFKBIKB
Gene Full Name Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Gene Summary
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-203 AL708460.1 9-211 204-3077 AF080158.1 170-3043 3078-3916 AK023193.1 1980-2818
Alternative Names
FLJ40509
IKK-beta
IKK2
IKKB
MGC131801
NFKBIKB
Molecular Weight(MW) 86kDa
Sequence 756 amino acids

Database Links

Entrez Gene 3551
SwissProt ID O14920
Protein Accession NP_001547

Application

  • Immunoblot

    Lane 1: 10ug 293T lysate (empty vector)
    Lane 2: 10ug IKKalpha-V5 transfected 293T lysate
    Lane 3: 10ug IKKbeta-V5 transfected 293T
    Primary Antibody Dilution:
    1:1000
    Secondary Antibody:
    Anti-rabbit HRP
    Secondary Antibody Dilution:
    1:2000
    Gene Name:
    IKBKB
    Submitted by:
    Dr. Tencho Tenev, The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research

  • Western blot

    0.2-1 ug/ml
    ELISA Titer: 1:312500
    Positive Control: Human brain

Application Notes
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM105584
  • Price: $336.00
  • Discount Price
  • Size:
  • Store: In stock
  • Lead time: 2~3 Week
  • Quantity:
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