All products Antibodies Raf-B Antibody (C-19) FITC

Raf-B Antibody (C-19) FITC

Catalog Number:OM160731

  • Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.

  • Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.

  • Western blot analysis of Raf-B expression in PC-12 whole cell lysate.

Product Profile

Product Name Raf-B Antibody (C-19) FITC
Antibody Type Primary Antibodies
Modification Notes Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFFCELISA

recommended for detection of Raf-B of mouse, rat and human origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope mapping at the C-terminus of Raf-B of human origin
Tissue Specificity epitope mapping at the C-terminus of Raf-B of human origin

Database Links

Entrez Gene 673

Application

  • Application

    Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization using indirect FITC (A) staining and HeLa cells using direct Alexa Fluor 488 (B) staining.

  • Application

    Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic staining.

  • Application

    Western blot analysis of Raf-B expression in PC-12 whole cell lysate.

Application Notes
recommended for detection of Raf-B of mouse, rat and human origin by WB, IP, IF, FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM160731
  • Price: $336.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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