All products Antibodies Syk Antibody (N-19) AC

Syk Antibody (N-19) AC

Catalog Number:OM152830

  • Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of squamous epithelial cells.

  • Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Western blot analysis of Syk expression in BJAB (A), Ramos (B), NAMALWA (C) and RAW 264.7 (D) whole cell lysates.

  • Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic staining.

Product Profile

Product Name Syk Antibody (N-19) AC
Antibody Type Primary Antibodies
Modification Notes Syk (spleen tyrosine kinase) is a 635 amino acid protein that contains one protein kinase domain and two SH2 domains. One of several members of the protein kinase superfamily, Syk functions as a positive effector of B cell antigen receptor (CD79)-stimulated responses, coupling CD79 with the movement of one calcium ion through one of two phospho-regulated pathways. Specifically, calcium ions travel through either a phosphoinositide 3-kinase (PI 3-kinase)-dependent pathway when Syk is not phosphorylated, or through a phospholipase C (PLC) ©-dependent pathway when human Syk is phohsphorylated on Tyr 348 and Tyr 352. Via its ability to influence CD79 activity and to control the movement of calicum through the cell, Syk plays an important role in a variety of cellular responses, including differentiation, phagocytosis, proliferation and B cell development. Syk expression is upregulated in T cell lymphoma, suggesting a possible role for Syk in tumorigenesis. Two isoforms of Syk, designated short and long, exist due to alternative splicing events.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFIHCFCELISA

WB:1:500~1:2000
IP:1:100~1:500
IF:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope mapping at the N-terminus of Syk of human origin
Tissue Specificity epitope mapping at the N-terminus of Syk of human origin

Database Links

Entrez Gene 6850

Application

  • Application

    Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of squamous epithelial cells.

  • Application

    Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Western blot analysis of Syk expression in BJAB (A), Ramos (B), NAMALWA (C) and RAW 264.7 (D) whole cell lysates.

  • Application

    Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic staining.

Application Notes
WB:1:500~1:2000
IP:1:100~1:500
IF:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM152830
  • Price: $336.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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