All products Antibodies c-Myc Antibody (A-14) Alexa Fluor® 488

c-Myc Antibody (A-14) Alexa Fluor® 488

Catalog Number:OM209453

  • Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer tissue showing nuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

  • Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Antibodies tested include c-Myc (A-14): (A,B) and c-Myc (A-14)-G: (C,D).

  • Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining.

  • Intracellular FCM analysis of fixed and permeabilized HL-60 cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Western blot analysis of whole cell lysates prepared from COS cells transfected with a c-Myc fusion protein (A) and non-transfected (B) cells.

  • Intracellular FCM analysis of methanol permeabilized COS cells transfected with c-Myc. Solid black line histogram represents control goat IgG.

  • Intracellular FCM analysis of methanol permeabilized HeLa cells. Solid black line histogram represents control goat IgG.

Product Profile

Product Name c-Myc Antibody (A-14) Alexa Fluor® 488
Antibody Type Tags Antibodies
Modification Notes c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFIHCFCELISAGSChIP

recommended for detection of c-Myc p67 and 9E10 Myc tagged fusion proteins of human and monkey origin by WB, IP, IF, IHC(P), FCM and ELISA; only minimal cross-reactivity with c-Myc of mouse and rat origin:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope mapping near the C-terminus of c-Myc of human origin
Tissue Specificity epitope mapping near the C-terminus of c-Myc of human origin

Database Links

Entrez Gene 4609

Application

  • Application

    Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervical cancer tissue showing nuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

  • Application

    Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Antibodies tested include c-Myc (A-14): (A,B) and c-Myc (A-14)-G: (C,D).

  • Application

    Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining.

  • Application

    Intracellular FCM analysis of fixed and permeabilized HL-60 cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Western blot analysis of whole cell lysates prepared from COS cells transfected with a c-Myc fusion protein (A) and non-transfected (B) cells.

  • Application

    Intracellular FCM analysis of methanol permeabilized COS cells transfected with c-Myc. Solid black line histogram represents control goat IgG.

  • Application

    Intracellular FCM analysis of methanol permeabilized HeLa cells. Solid black line histogram represents control goat IgG.

Application Notes
recommended for detection of c-Myc p67 and 9E10 Myc tagged fusion proteins of human and monkey origin by WB, IP, IF, IHC(P), FCM and ELISA; only minimal cross-reactivity with c-Myc of mouse and rat origin:

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM209453
  • Price: $336.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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