Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Western blot analysis of c-Myc expression in non-transfected: (A) and mouse c-Myc transfected: (B) 293T whole cell lysates.
Western blot analysis of c-Myc expression in Jurkat whole cell lysate.
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse uterus tissue showing nuclear localization.
Product Name | c-Myc Antibody (C-19) |
---|---|
Antibody Type | Tags Antibodies |
Modification Notes | c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3. |
Clonality | Polyclonal |
---|---|
Isotype | IgG |
Host Species | Rabbit |
Tested Applications | |
recommended for detection of c-Myc p67 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine and feline: |
|
Species Reactivity | |
Concentration | 1mg/ml |
Purification | Affinity purified |
Alternative Names | epitope mapping within the C-terminus of c-Myc of human origin |
---|---|
Tissue Specificity | epitope mapping within the C-terminus of c-Myc of human origin |
Entrez Gene | 4609 |
---|
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Western blot analysis of c-Myc expression in non-transfected: (A) and mouse c-Myc transfected: (B) 293T whole cell lysates.
Western blot analysis of c-Myc expression in Jurkat whole cell lysate.
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse uterus tissue showing nuclear localization.
Application Notes | recommended for detection of c-Myc p67 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine and feline: |
---|
Form | Liquid |
---|---|
Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
2013 © Omnimabs , All Rights Reserved.