All products Antibodies p53 Antibody (FL-393) FITC

p53 Antibody (FL-393) FITC

Catalog Number:OM152338

  • Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of squamous epithelial cells.

  • Western blot analysis of p53 expression in Lac-Z whole cell lysate. Antibody tested: p53 (FL-393): .

  • Western blot analysis of p53 expression in Jurkat whole cell lysate. Antibody tested: p53 (FL-393)-G: .

  • Western blot analysis of p53 expression in Lac-Z whole cell lysate. Antibody tested: p53 (FL-393)-G: .

  • Western blot analysis of p53 expression in untreated (A) and Farnesyl Thiosalicylic Acid Amide treated (B) SW480 whole cell lysates. Note down regulation of p53 expression in lane B.

  • Western blot analysis of p53 expression in A-431 (A), BC3H1 (B), MCP-5 (C), Lac Z (D) and WR19L (E) whole cell lysates.

  • Western blot analysis of p53 expression in non-transfected: (A) and human p53 transfected: (B) 293T whole cell lysates.

  • Western blot analysis of p53 expression in non-transfected: (A) and mouse p53 transfected: (B) 293T whole cell lysates.

  • Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal goat IgG: .

  • Immunofluorescence staining of methanol-fixed, control HeLa (A) and p53 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with p53 (FL-393): .

  • Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Western blot analysis of p53 expression in A-431 (A), WR19L (B) and mouse Lac Z (C) whole cell lysates.

  • Immunofluorescence staining of methanol-fixed A-431 cells showing nuclear localization.

  • Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue. Note nuclear staining of ductal epithelial.

Product Profile

Product Name p53 Antibody (FL-393) FITC
Antibody Type Primary Antibodies
Modification Notes p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WBIPIFIHCFCELISA

WB:1:500~1:2000
IP:1:100~1:500
IF:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Alternative Names
epitope mapping within amino acids 1-393 representing full length p53 of human origin
Tissue Specificity epitope mapping within amino acids 1-393 representing full length p53 of human origin

Database Links

Entrez Gene 7157

Application

  • Application

    Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of squamous epithelial cells.

  • Application

    Western blot analysis of p53 expression in Lac-Z whole cell lysate. Antibody tested: p53 (FL-393): .

  • Application

    Western blot analysis of p53 expression in Jurkat whole cell lysate. Antibody tested: p53 (FL-393)-G: .

  • Application

    Western blot analysis of p53 expression in Lac-Z whole cell lysate. Antibody tested: p53 (FL-393)-G: .

  • Application

    Western blot analysis of p53 expression in untreated (A) and Farnesyl Thiosalicylic Acid Amide treated (B) SW480 whole cell lysates. Note down regulation of p53 expression in lane B.

  • Application

    Western blot analysis of p53 expression in A-431 (A), BC3H1 (B), MCP-5 (C), Lac Z (D) and WR19L (E) whole cell lysates.

  • Application

    Western blot analysis of p53 expression in non-transfected: (A) and human p53 transfected: (B) 293T whole cell lysates.

  • Application

    Western blot analysis of p53 expression in non-transfected: (A) and mouse p53 transfected: (B) 293T whole cell lysates.

  • Application

    Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal goat IgG: .

  • Application

    Immunofluorescence staining of methanol-fixed, control HeLa (A) and p53 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with p53 (FL-393): .

  • Application

    Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .

  • Application

    Western blot analysis of p53 expression in A-431 (A), WR19L (B) and mouse Lac Z (C) whole cell lysates.

  • Application

    Immunofluorescence staining of methanol-fixed A-431 cells showing nuclear localization.

  • Application

    Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue. Note nuclear staining of ductal epithelial.

Application Notes
WB:1:500~1:2000
IP:1:100~1:500
IF:1:200~1:500
IHC:1:50~1:200
FC:1:200~1:500
Notes:Optimal dilutions/concentrations should be determined by the researcher.

Additional Information

Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

  • Catalog Number: OM152338
  • Price: $336.00
  • Discount Price
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  • Store: Out of stock
  • Lead time: 4~6 Week
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