Proper dissolution is an important factor in the success of peptide experiments. Inappropriate dissolving can cause loss of peptides and failure of experiments; it is suggested that a small number of peptides be used to test the optimal solution (small test), only when the polypeptide is completely dissolved, the solution can be added and diluted to the final concentration. Usually, distilled water or sterile water is used for small tests, especially for oligopeptides with a length of less than five amino acids. For other special peptides, it is necessary to optimize the dissolution conditions according to the sequence of peptides.
The dissolution of freeze-dried polypeptide
Guide to dissolution test:
1. each acid amino acid in the polypeptide sequence includes aspartic acid Asp (D), glutamic acid Glu (E) and the carboxyl -COOH of C terminal is -1, each basic amino acid includes arginine Arg (R), lysine Lys (K), histidine His and amino acids of 0. Based on this, the value of the whole polypeptide is calculated. The positive score is called the basic polypeptide. The negative score is called acidic polypeptide, and the zero score is called neutral polypeptide.
2. if it is alkaline peptide, first try to dissolve with deionized water; if insoluble, try to use 10% - 30% acetic acid; if the polypeptide is insoluble, try to use pure acetic acid and three FLUOROACETIC acid TFA (
Key Feature
Isotype
Cocktail
Host Species
Alligator
Purification
Protein A
Target Information
Gene Symbol
β-Amyloid Peptide (1-42)
Additional Information
Storage Instructions
-20°C
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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