Application
A. Western blot analysis on T47D cell lysate using anti-MUC1 RabMAb (Cat. #AJ1517a), dilution 1:1000.Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-MUC1 RabMAb (Cat. #AJ1517a).| Product Name | MUC1 Antibody (isoform 7) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MUC1, PUM, Mucin-1 subunit beta, MUC1-CT |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP1024Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PUM |
|---|---|
| Alternative Names | MUC1 PUM Mucin-1 subunit beta MUC1-CT |
| Molecular Weight(MW) | 122102 Da |
| Function | The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack |
| Tissue Specificity | Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only |
| Cellular Localization | Apical cell membrane; Single-pass type I membrane protein. Note=Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane Located to microvilli and to the tips of long filopodial protusions Isoform 7: Secreted. Mucin-1 subunit beta: Cell membrane. Cytoplasm. Nucleus. Note=On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus |
| Entrez Gene | 4582 |
|---|
Application
A. Western blot analysis on T47D cell lysate using anti-MUC1 RabMAb (Cat. #AJ1517a), dilution 1:1000.
Application
B. Immunohistochemical staining of paraffin-embedded human breast carcinoma using anti-MUC1 RabMAb (Cat. #AJ1517a).| Application Notes | WB:1:1000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Data sheet for OM214772