Application
Dot blot analysis of anti-Phospho-MUC1-pT1224 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.Application
Confocal immunofluorescent analysis of Phospho-MUC1-T1224 Antibody(Cat#AP3728a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Product Name | Phospho-MUC1- T1224 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DBIF |
| DB:1:500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PUM |
|---|---|
| Alternative Names | MUC1 PUM Mucin-1 Breast carcinoma-associated antigen DF3 Carcinoma-associated mucin Episialin H23AG Krebs von den Lungen-6 PEMT Peanut-reactive urinary mucin Polymorphic epithelial mucin Tumor-associated epithelial membrane antigen Tumor-associ |
| Molecular Weight(MW) | 122102 Da |
| Function | The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack |
| Tissue Specificity | Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only |
| Cellular Localization | Apical cell membrane; Single-pass type I membrane protein. Note=Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized and recycled to the cell membrane Located to microvilli and to the tips of long filopodial protusions Isoform 7: Secreted. Mucin-1 subunit beta: Cell membrane. Cytoplasm. Nucleus. Note=On EGF and PDGFRB stimulation, transported to the nucleus through interaction with CTNNB1, a process which is stimulated by phosphorylation. On HRG stimulation, colocalizes with JUP/gamma-catenin at the nucleus |
| Entrez Gene | 4582 |
|---|
Application
Dot blot analysis of anti-Phospho-MUC1-pT1224 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Application
Confocal immunofluorescent analysis of Phospho-MUC1-T1224 Antibody(Cat#AP3728a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).| Application Notes | DB:1:500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
Data sheet for OM217540