Application
Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-TBB5 Antibody (Cat. #AM1031a) in Y79,CEM,293 cell line and mouse brain tissue lysates (35ug/lane). TBB5(arrow) was detected using the purified Mab.(1ug/ml)Application
Confocal immunofluorescent analysis of TBB5 Antibody (Cat. #AM1031a) with Hela cell followed by PE-conjugated goat anti-mouse lgG (red). DAPI was used to stain the cell nuclear (blue).| Product Name | TBB5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TUBB; TUBB5; Tubulin beta chain; Tubulin beta-5 chain |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 87CT59.3.7 |
| Host Species | Mouse |
| Tested Applications | IHCWBIF |
| IHC:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TUBB5 |
|---|---|
| Alternative Names | TUBB TUBB5 Tubulin beta chain Tubulin beta-5 chain |
| Molecular Weight(MW) | 49671 Da |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain |
| Tissue Specificity | Ubiquitously expressed with highest levels in spleen, thymus and immature brain |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 203068 |
|---|
Application
Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-TBB5 Antibody (Cat. #AM1031a) in Y79,CEM,293 cell line and mouse brain tissue lysates (35ug/lane). TBB5(arrow) was detected using the purified Mab.(1ug/ml)
Application
Confocal immunofluorescent analysis of TBB5 Antibody (Cat. #AM1031a) with Hela cell followed by PE-conjugated goat anti-mouse lgG (red). DAPI was used to stain the cell nuclear (blue).| Application Notes | IHC:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Data sheet for OM222605