Application
Western blot analysis of TBB5 Antibody (C-term) in A375, A2058 cell line lysates (35ug/lane). TBB5 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human colon carcinoma with TBB5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of WiDr cells using TBB5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TBB5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TUBB; TUBB5; Tubulin beta chain; Tubulin beta-5 chain |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TUBB5 |
|---|---|
| Alternative Names | TUBB TUBB5 Tubulin beta chain Tubulin beta-5 chain |
| Molecular Weight(MW) | 49671 Da |
| Function | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain |
| Tissue Specificity | Ubiquitously expressed with highest levels in spleen, thymus and immature brain |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Entrez Gene | 203068 |
|---|
Application
Western blot analysis of TBB5 Antibody (C-term) in A375, A2058 cell line lysates (35ug/lane). TBB5 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma with TBB5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of WiDr cells using TBB5 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM222611