| Product Name | CXCL8 / IL8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [6217] to IL8IL8, formerly called monocyte derived neutrophil chemotactic factor, belongs to the chemokine alpha family. Human endothelial IL8 is an 8.9 kDa protein containing 77 amino acid residues. IL8 exhibits chemotactic activity in vitro for T cells, basophils and neutrophils and promotes degranulation. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 6217 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSNeutWB |
| ELISA: Use at a concentration of 4 μg/ml. FACS: Use at a concentration of 25 μg/ml. Neut: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 11 kDa. Monoclonal Anti-Interleukin 8 has the ability to neutralize human IL8 bioactivity. The ND50 for this effect is approximately 0.08-0.4μg/ml in the presence of 0.02μg/ml of recombinant human IL8 using hCXCR-2 transfected BaF/3 cells. The exact concentration of antibody required to neutralize recombinant human IL8 activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity. The detection limit for in immunoblotting for recombinant human IL8 is approximately 0.5 ng/lane under non-reducing and reducing conditions in SDS PAGE. For capture ELISAs, monoclonal anti-interleukin 8 can be used as the capture antibody. A working concentration of the capture antibody at 4μg/ml (100μl/well) in combination with a biotinylated detection antibody at 20 ng/ml (100μl/well) is recommended to detect human IL8. For flow cytometry (intracellular staining), the cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. The cells (100,000) are stained with the primary antibody using 10μl of a 25μg/ml stock solution. Following a 30 minute incubation, the cells should be washed with 0.1% saponin prior to using a fluorochrome conjugated secondary reagent for detection (typically, 250 ng of a polyclonal antibody per reaction is added to develop the staining). After incubation with the fluorochrome, the cells should be washed for a final time in 0.1% saponin prior to analysis. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | CXCL8 GCP-1 GCP1 LECT LUCT LYNAP MDNCF MONAP NAF NAP-1 NAP1 GCP 1 NAP 1 |
|---|---|
| Target | IL8 |
| Tissue Specificity | By immunoblotting, the antibody shows less than 1% cross-reactivity with recombinant human GRO-beta under reducing conditions and less than 1% cross-reactivity with recombinant mouse JE under non-reducing conditions. |
| Cellular Localization | Secreted |
| Application Notes | ELISA: Use at a concentration of 4 μg/ml. FACS: Use at a concentration of 25 μg/ml. Neut: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 11 kDa. Monoclonal Anti-Interleukin 8 has the ability to neutralize human IL8 bioactivity. The ND50 for this effect is approximately 0.08-0.4μg/ml in the presence of 0.02μg/ml of recombinant human IL8 using hCXCR-2 transfected BaF/3 cells. The exact concentration of antibody required to neutralize recombinant human IL8 activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity. The detection limit for in immunoblotting for recombinant human IL8 is approximately 0.5 ng/lane under non-reducing and reducing conditions in SDS PAGE. For capture ELISAs, monoclonal anti-interleukin 8 can be used as the capture antibody. A working concentration of the capture antibody at 4μg/ml (100μl/well) in combination with a biotinylated detection antibody at 20 ng/ml (100μl/well) is recommended to detect human IL8. For flow cytometry (intracellular staining), the cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin. The cells (100,000) are stained with the primary antibody using 10μl of a 25μg/ml stock solution. Following a 30 minute incubation, the cells should be washed with 0.1% saponin prior to using a fluorochrome conjugated secondary reagent for detection (typically, 250 ng of a polyclonal antibody per reaction is added to develop the staining). After incubation with the fluorochrome, the cells should be washed for a final time in 0.1% saponin prior to analysis. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: PBS containing 5% Trehalose. |
Data sheet for OM248643