Application
Antibody used in WB on Human Jurkat 0.0625 ug/ml.| Product Name | HSF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSF1, HSTF1 |
| Product description | HSF1 is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1.HSF1 is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1.1) Wang, J., et al., (2006) J. Biol. Chem. 281 (5), 2451-2459. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| HSF1 antibody can be used for detection of HSF1 by ELISA at 1:12500. HSF1 antibody can be used for detection of HSF1 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSF1 |
|---|---|
| Alternative Names | HSF1 HSTF1 |
| Molecular Weight(MW) | 57 kDa, 56 kDa, 56 kDa |
Application
Antibody used in WB on Human Jurkat 0.0625 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | HSF1 antibody can be used for detection of HSF1 by ELISA at 1:12500. HSF1 antibody can be used for detection of HSF1 by western blot at 0.0625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store HSF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM281361