Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 (Ab-303).Application
Western blot analysis of lysed extracts from MCF7 cells using HSF1 (Ab-303).| Product Name | HSF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSTF1, CMKBR7, EBI1, EVI1, HSTF 1 |
| Product description | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.1) Parvaneh Rafiee, et, al. (2006) Am J Physiol Cell Physiol ; 291: C931 - C945Fumika Shinozaki, et, al. (2006) J. Biol. Chem ; 281: 16361 - 16369. Eiichi Takaki, et, al. (2006) J. Biol. Chem ; 281: 4931 - 4937. Jan-Jong Hung, et, al. (1998) J. Biol. Chem ; 273: 31924. |
| Immunogen | HSF1 (Ab-303) antibody was raised against a peptide sequence around aa.301~305 (P-P-S-P-P) derived from Human HSF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSF1 |
|---|---|
| Alternative Names | HSTF1 CMKBR7 EBI1 EVI1 HSTF 1 |
| Molecular Weight(MW) | 82 kDa |
| Tissue Specificity | This antibody detects endogenous level of total HSF1 H3.1 protein. |
| Entrez Gene | 3297 |
|---|---|
| Protein Accession | NP_005517.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 (Ab-303).
Application
Western blot analysis of lysed extracts from MCF7 cells using HSF1 (Ab-303).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM281357