HSF1 mediates the stress induced expression of heat shock proteins.1) Shamovsky, I., et al., (2006) Nature 440 (7083), 556-560.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HSF1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
HSF1 antibody can be used for detection of HSF1 by ELISA at 1:62500. HSF1 antibody can be used for detection of HSF1 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse NIH-3T3 at 5.0 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
HSF1 antibody can be used for detection of HSF1 by ELISA at 1:62500. HSF1 antibody can be used for detection of HSF1 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HSF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt