Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using HSF1 (Phospho-Thr142) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | HSF1 (phospho Thr142) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HSF 1, HSTF1, Heat shock factor protein 1 |
| Product description | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.1) Rabindran S.K., Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910(1991). |
| Immunogen | HSF1 (phospho Thr142) antibody was raised against a peptide sequence around phosphorylation site of threonine 142 (L-L-T (p)-D-V) derived from Human HSF1 . |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSF1 |
|---|---|
| Alternative Names | HSF 1 HSTF1 Heat shock factor protein 1 |
| Molecular Weight(MW) | 57 kDa |
| Tissue Specificity | The antibody detects endogenous levels of HSF1 only when phosphorylated at threonine 142. |
| Entrez Gene | 3297 |
|---|---|
| Protein Accession | NP_005517.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using HSF1 (Phospho-Thr142) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM281358