Application
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with KPNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of KPNB1 Antibody in Y79 cell line lysates (35ug/lane)| Product Name | KPNB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Importin subunit beta-1, Importin-90, Karyopherin subunit beta-1, Nuclear factor p97, Pore targeting complex 97 kDa subunit, PTAC97, KPNB1, NTF97 |
| Product description | NTF97 is involved in nuclear protein import, either by associating itself with an adapter protein (for example, importin-alpha subunit which binds to nuclear localization signals (NLS) in cargo substrates), or by acting autonomously as a nuclear transport receptor.1) Nordgard,S.H., et.al., Genes Chromosomes Cancer 47 (8), 680-696 (2008) |
| Immunogen | This KPNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-216 amino acids from the N-terminal region of human KPNB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | KPNB1 |
|---|---|
| Alternative Names | Importin subunit beta-1 Importin-90 Karyopherin subunit beta-1 Nuclear factor p97 Pore targeting complex 97 kDa subunit PTAC97 KPNB1 NTF97 |
| Molecular Weight(MW) | 97 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with KPNB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of KPNB1 Antibody in Y79 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM283063