| Product Name | PARP-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. |
| Immunogen | Recombinant human PARP-1 [ARTD1] (aa 215-525). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Immunocytochemistry: 1:500-1:1,000 . Immunohistochemistry: 1:500-1:1,000 . Immunoprecipitation: 2ul per 100ug nuclear extract Western Blot: 1:2,000-1:5,000 . Optimal conditions must be determined individually for each application. | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Symbol | PARP11 |
|---|---|
| Alternative Names | Poly(ADP-ribose) Polymerase 1 ADP-ribosyltransferase Diphtheria Toxin-like 1 NAD(+) ADP-ribosyltransferase 1 |
| Tissue Specificity | Recognizes human PARP-1. Cross-reacts weakly with mouse and monkey PARP-1. |
| Application Notes | Immunocytochemistry: 1:500-1:1,000 . Immunohistochemistry: 1:500-1:1,000 . Immunoprecipitation: 2ul per 100ug nuclear extract Western Blot: 1:2,000-1:5,000 . Optimal conditions must be determined individually for each application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 1 year after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. Contains 0.1% sodium azide. |
Data sheet for OM287371