WB
Western blot analysis of extracts of various cell lines, using UGT1A6 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.ICC/IF
Immunofluorescence analysis of mouse liver using UGT1A6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.| Product Name | UGT1A6 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 65-270 of human UGT1A6 (NP_001063.2). |
| Clonality | polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | UGT1A6 |
|---|---|
| Gene Synonyms | GNT1 UGT1 HLUGP UDPGT UGT1A UGT1C UGT1E UGT1F HLUGP1 UGT-1A UGT-1C UGT-1E UGT-1F UGT1.1 UGT1.3 UGT1.5 UGT1.6 UGT1A1 UGT1A3 UGT1A5 UGT1-01 UGT1-03 UGT1-05 UGT1-06 UGT1A6S hUG-BR1 UDPGT 1-6 |
| Gene Full Name | UDP glucuronosyltransferase family 1 member A6 |
| Gene Summary | This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenolic and planar compounds. Alternative splicing in the unique 5' end of this gene results in two transcript variants. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 61kDa(Observed MW 70kDa) |
| Cellular Localization | Endoplasmic reticulum membrane, Microsome, Single-pass membrane protein. |
WB
Western blot analysis of extracts of various cell lines, using UGT1A6 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
ICC/IF
Immunofluorescence analysis of mouse liver using UGT1A6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
Data sheet for OM644262