Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MyoD (Phospho-Ser200).| Product Name | MyoD (phospho Ser200) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and themyogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite formyogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.1) Emmanuel G, et al. J. Biol. Chem., Jun 2000; 275: 18767 - 18776Tintignac LA, et al. Mol Cell Biol. 2004 Feb; 24 (4): 1809-1821. Kitzmann M, et a. Mol Cell Biol. 1999 Apr; 19 (4): 3167-3176. |
| Immunogen | MyoD (Phospho-Ser200) antibody was raised against a peptide sequence around phosphorylation site of serine 200 (A-S-S (p) -P-R) derived from Human MyoD. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYOD1 |
|---|---|
| Alternative Names | PUM MYF3 MYOD bHLHc1 IBP3 MYOD1 |
| Molecular Weight(MW) | 40 kDa |
| Tissue Specificity | This antibody detects endogenous level of MyoD only when phosphorylated at serine 200. |
| Entrez Gene | 4654 |
|---|---|
| Protein Accession | NP_002469.2 |
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF using MyoD (Phospho-Ser200).| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM285683