Application
Immunohistochemistry staining of PLCG1 in colon, lymphocytes: formalin-fixed, paraffin-embedded (ffpe)PLCG1 Antibody.| Product Name | PLCG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLCG1, Phospholipase C, gamma 1, Phospholipase C-148, Phosphoinositidase C, Phospholipase C-II, Inositoltrisphosphohydrolase, PLC-gamma-1, PLC1, PLC-148, PLC-II, PLC148, NCKAP3, Phospholipase C-gamma-1, PLCgamma1 |
| Product description | Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5- triphosphate (IP3) and diacylglycerol (DAG). At least four families of PLC5, PLCbeta, PLCgamma, PLCdelta and PLCepsilon have been identified so far. The PLCbeta subfamily includes four members, PLCbeta1-4. All four members of the subfamily are activated by alpha-or beta-gamma-subunits of the heterotrimeric G-proteins (2,3). Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of serine 1105 by PKA or PKC inhibits PLCbeta3 activity (4,5). Serine 537 of PLCbeta3 is phosphorylated by CaMKII and this phosphorylation may contribute to the basal activity of PLCbeta3. PLCgamma is activated by both receptor and nonreceptor tyrosine kinases. PLCgamma forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCgamma1 on tyrosine residues 771, 783 and 1245. Phosphorylation by Syk at tyrosine 783 activates the enzymatic activity of PLCgamma1 (8). Detects total human PLCgamma1 protein levels. Does not crossreact with PLCgamma2. Species Crossreactivity: Mouse. Predicted to crossreact with rat based on sequence homology. |
| Immunogen | PLCG1 antibody was raised against a synthetic peptide corresponding to the C-Terminal residues of PLCG1 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| PLCG1 antibody can be used in immunohistochemistry starting at 1:50.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PLCG1 |
|---|---|
| Alternative Names | PLCG1 Phospholipase C gamma 1 Phospholipase C-148 Phosphoinositidase C Phospholipase C-II Inositoltrisphosphohydrolase PLC-gamma-1 PLC1 PLC-148 PLC-II PLC148 NCKAP3 Phospholipase C-gamma-1 PLCgamma1 |
Application
Immunohistochemistry staining of PLCG1 in colon, lymphocytes: formalin-fixed, paraffin-embedded (ffpe)PLCG1 Antibody.| Application Notes | PLCG1 antibody can be used in immunohistochemistry starting at 1:50.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store PLCG1 antibody should be stored at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50% glycerol. No preservative added. |
Data sheet for OM288284