Application
Western blot analysis in CEM cell lysate (35ug/lane). This demonstrates that the detected the POLH protein (arrow).| Product Name | POLH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA polymerase eta, RAD30 homolog A, Xeroderma pigmentosum variant type protein, POLH, RAD30, RAD30A, XPV |
| Product description | DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.1) Masutani C.,et al.Nature 399:700-704(1999). Johnson R.E.,et al.Science 285:263-265(1999). Yuasa M.,et al.Oncogene 19:4721-4728(2000). Mungall A.J.,et al.Nature 425:805-811(2003). Glick E.,et al.EMBO J. 20:7303-7312(2001). |
| Immunogen | This POLH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 68-92 amino acids from the N-terminal region of human POLH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLH |
|---|---|
| Alternative Names | DNA polymerase eta RAD30 homolog A Xeroderma pigmentosum variant type protein POLH RAD30 RAD30A XPV |
| Molecular Weight(MW) | 78 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in CEM cell lysate (35ug/lane). This demonstrates that the detected the POLH protein (arrow).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM288443