Application
Western blot analysis of TOR in L1210 cell lysate with TOR antibody at (A) 1 and (B) 2 μg/mL.Application
Immunocytochemistry of TOR in L1210 cells with TOR antibody at 2 μg/mL.| Product Name | TOR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TOR Antibody: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, FRAP, FK506-binding protein 12-rapamycin complex-associated protein 1, mTOR |
| Product description | TOR Antibody: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin. Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. TOR is normally associated with the regulatory proteins RAPTOR and GbetaL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis.1) Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80. |
| Immunogen | TOR antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TOR. The immunogen is located within amino acids 210 - 260 of TOR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| TOR antibody can be used for the detection of TOR by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FRAP1 |
|---|---|
| Alternative Names | TOR Antibody: FRAP FRAP1 FRAP2 RAFT1 RAPT1 FRAP FK506-binding protein 12-rapamycin complex-associated protein 1 mTOR |
| Sequence Similarities | 2) Cat. No. 17-204 - L1210 Cell Slide |
Application
Western blot analysis of TOR in L1210 cell lysate with TOR antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunocytochemistry of TOR in L1210 cells with TOR antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1284 - L1210 Cell Lysate |
|---|---|
| Application Notes | TOR antibody can be used for the detection of TOR by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TOR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TOR Antibody is supplied in PBS containing 0.02% sodium azide. |
Data sheet for OM293769