WB
Western blot analysis using NLRP3 antibody against Hep G2 (1), K562 (2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using NLRP3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using NLRP3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using NLRP3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using NLRP3 antibody with DAB staining.WB
(C-E) The protein levels of ERα, NLRP3, ASC and Casp-1p20 in NCM-460 cells following different drug treatments.WB
(F) The protein levels of ERα and NLRP3 in colon tissues.WB
(B) The protein level of ERα and NLRP3 in ERα-overexpressing cellsWB
(B) Co-immunoprecipitations of ERα and NLRP3/pro-casp-1/ASC complexes.WB
(E-F) Western blot results of NLRP3, ER, Wnt2b, and β-catenin different cells| Product Name | Anti-NLRP3 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NLRP3 |
|---|---|
| Gene Synonyms | AII AVP FCU MWS FCAS KEFH CIAS1 FCAS1 NALP3 C1orf7 CLR1.1 DFNA34 PYPAF1 AGTAVPRL |
| Gene Full Name | NLR family pyrin domain containing 3 |
| Gene Summary | This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Aug 2020] |
| Molecular Weight(MW) | 118 kDa |
| Function | As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (and possibly CASP4 and CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu |
| Cellular Localization | Cytosol;Extracellular region or secreted;Endoplasmic reticulum;Nucleus |
| SwissProt ID | Q96P20 |
|---|
WB
Western blot analysis using NLRP3 antibody against Hep G2 (1), K562 (2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using NLRP3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using NLRP3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using NLRP3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using NLRP3 antibody with DAB staining.
WB
(C-E) The protein levels of ERα, NLRP3, ASC and Casp-1p20 in NCM-460 cells following different drug treatments.
WB
(F) The protein levels of ERα and NLRP3 in colon tissues.
WB
(B) The protein level of ERα and NLRP3 in ERα-overexpressing cells
WB
(B) Co-immunoprecipitations of ERα and NLRP3/pro-casp-1/ASC complexes.
WB
(E-F) Western blot results of NLRP3, ER, Wnt2b, and β-catenin different cells| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
