WB
Western blot analysis using GRP78 antibody against Hela (1), NIH 3T3 (2), Hep G2 (3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human cervica cancer tissues using GRP78 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using GRP78 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using GRP78 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using GRP78 antibody with DAB staining.| Product Name | Anti-GRP78 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | HSPA5 |
|---|---|
| Gene Synonyms | BIP HSPA5 HEL-S-89n |
| Gene Full Name | heat shock protein family A (Hsp70) member 5 |
| Gene Summary | The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers. [provided by RefSeq, Jul 2020] |
| Molecular Weight(MW) | 78 kDa（Observed:54 kDa) |
| Source | Rabbit |
| Cellular Localization | Cytoplasm; Endoplasmic reticulum |
| SwissProt ID | P11021 |
|---|
WB
Western blot analysis using GRP78 antibody against Hela (1), NIH 3T3 (2), Hep G2 (3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human cervica cancer tissues using GRP78 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using GRP78 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using GRP78 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using GRP78 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azi. |
