WB
Western blot analysis using Caspase 8 antibody against THP-1 (1), MG-63 (2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using Caspase 8 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using Caspase 8 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using Caspase 8 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using Caspase 8 antibody with DAB staining.| Product Name | Anti-Caspase 8 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP8 |
|---|---|
| Gene Synonyms | CAP4 MACH MCH5 FLICE ALPS2B Casp-8 |
| Gene Full Name | caspase 8 |
| Gene Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 55 kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm |
| SwissProt ID | Q14790 |
|---|
WB
Western blot analysis using Caspase 8 antibody against THP-1 (1), MG-63 (2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using Caspase 8 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using Caspase 8 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using Caspase 8 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using Caspase 8 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
