WB
Western blot analysis using c-Kit antibody against HEK293 (1), SK-OV-3 (2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using c-Kit antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using c-Kit antibody with DAB staining.| Product Name | Anti-c-Kit antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | KIT |
|---|---|
| Gene Synonyms | PBT SCFR C-Kit CD117 MASTC |
| Gene Full Name | KIT proto-oncogene, receptor tyrosine kinase |
| Gene Summary | This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2020] |
| Molecular Weight(MW) | 110 kDa |
| Source | Rabbit |
| Cellular Localization | Cell membrane and Cytoplasm |
| SwissProt ID | P10721 |
|---|
WB
Western blot analysis using c-Kit antibody against HEK293 (1), SK-OV-3 (2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissues using c-Kit antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using c-Kit antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
