ICC/IF
Immunofluorescence analysis of Hela cells using CA9 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Product Name | Anti-CA9 antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFFC |
| IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CA9 |
|---|---|
| Gene Synonyms | Ca9 CAIX MN MN/CA9 |
| Gene Full Name | carbonic anhydrase 9 |
| Gene Summary | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014] |
| Alternative Names | CA IX variant CA-9 CA-IX carbonate dehydratase IX carbonic anhydrase IX carbonic dehydratase membrane antigen MN membrane antigen MN homolog P54 / 58N P54/58N pMW1 RCC-associated antigen G250 RCC-associated protein G250 renal cell carcinoma-associated antigen G250 |
| Molecular Weight(MW) | 50KDa（observed：54-60kDa) |
| Source | Rabbit |
| Cellular Localization | Nucleus. Plasma membrane. |
| SwissProt ID | Q16790 |
|---|
ICC/IF
Immunofluorescence analysis of Hela cells using CA9 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Application Notes | IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
