ICC/IF
Immunofluorescence analysis of A431 cells using LEPR antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Product Name | Anti-LEPR antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFFC |
| IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LEPR |
|---|---|
| Gene Synonyms | B219 CD295 db diabetes Fa huB219 LEP-R Leprb LEPRD LEPROT Modb1 OB-R OB-RGRP obese-like obl OBR |
| Gene Full Name | leptin receptor |
| Gene Summary | The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010] |
| Alternative Names | CD antigen CD295 CD295 antigen cytokine receptor HuB219.2 LEP-R Leptin receptor (fatty) leptin receptor gene-related protein OB receptor OB-R OB-Rb cytokine receptor splice variant splice variant form 12.1 Ob receptor splice variant form 13.2 Ob receptor splice variant form 6.4 Ob receptor |
| Molecular Weight(MW) | 132 KDa(observed:50、120kDa） |
| Source | Rabbit |
| Cellular Localization | Secreted and Cell membrane. |
| SwissProt ID | P48357 |
|---|
ICC/IF
Immunofluorescence analysis of A431 cells using LEPR antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.| Application Notes | IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
