WB
Western blot analysis using CyclinE1 antibody against COS-7 (1), A549 (2), K562 (3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using CyclinE1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using CyclinE1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using CyclinE1 antibody with DAB staining.| Product Name | Anti-CyclinE1 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CCNE1 |
|---|---|
| Gene Synonyms | CCNE1 CCNE pCCNE1 |
| Gene Full Name | cyclin E1 |
| Gene Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016] |
| Molecular Weight(MW) | 47KDa |
| Source | Rabbit |
| Cellular Localization | Nucleus |
| SwissProt ID | P24864 |
|---|
WB
Western blot analysis using CyclinE1 antibody against COS-7 (1), A549 (2), K562 (3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using CyclinE1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using CyclinE1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using CyclinE1 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
