WB
Western blot analysis using BRAF antibody against NIH3T3 (1), HEK293 (2), C2C12 (3) and HepG2 (4) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using BRAF antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using BRAF antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast tissues using BRAF antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using BRAF antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using BRAF antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using BRAF antibody with DAB staining.| Product Name | Anti-BRAF antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinent Proteins |
| Conjugation | HRP |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BRAF |
|---|---|
| Gene Synonyms | NS7 B-raf BRAF1 RAFB1 B-RAF1 |
| Gene Full Name | B-Raf proto-oncogene, serine/threonine kinase |
| Gene Summary | This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017] |
| Molecular Weight(MW) | 84 kDa |
| Source | Mouse |
| Cellular Localization | Cell membrane, Cytoplasm, Membrane, Nucleus |
| SwissProt ID | P15056 |
|---|
WB
Western blot analysis using BRAF antibody against NIH3T3 (1), HEK293 (2), C2C12 (3) and HepG2 (4) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using BRAF antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using BRAF antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast tissues using BRAF antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using BRAF antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using BRAF antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using BRAF antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
