IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using CEBPB antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using CEBPB antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using CEBPB antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using CEBPB antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using CEBPB antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using CEBPB antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using CEBPB antibody with DAB staining.WB
Western blot analysis using CEBPBeta antibody against Mouse heart(1), Rat intestine(2),Rat stomach(3),Rat spleen(4)tissue. gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time| Product Name | Anti-CEBPB antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CEBPB |
|---|---|
| Gene Synonyms | TCF5 IL6DBP NF-IL6 C/EBP-beta C/EBPBeta |
| Gene Full Name | CCAAT enhancer binding protein beta |
| Gene Summary | This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. [provided by RefSeq, Oct 2013] |
| Molecular Weight(MW) | 36kDa |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | P17676 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissues using CEBPB antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using CEBPB antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using CEBPB antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using CEBPB antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using CEBPB antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissues using CEBPB antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using CEBPB antibody with DAB staining.
WB
Western blot analysis using CEBPBeta antibody against Mouse heart(1), Rat intestine(2),Rat stomach(3),Rat spleen(4)tissue. gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
