WB
Western blot analysis using MEKK3 antibody against K562(1),Thp-1(2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using MEKK3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using MEKK3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using MEKK3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human kidney tissues using MEKK3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using MEKK3 antibody with DAB staining.| Product Name | Anti-MEKK3 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP3K3 |
|---|---|
| Gene Synonyms | MEKK3 MAPKKK3 |
| Gene Full Name | mitogen-activated protein kinase kinase kinase 3 |
| Gene Summary | This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 71 kDa |
| Cellular Localization | Cytosol, cytoplasm |
| SwissProt ID | Q99759 |
|---|
WB
Western blot analysis using MEKK3 antibody against K562(1),Thp-1(2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using MEKK3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using MEKK3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using MEKK3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human kidney tissues using MEKK3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using MEKK3 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
