WB
Western blot analysis using PARK7 antibody against Hela(1), Thp-1(2), MCF-7(3), COS-7(4) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PARK7 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PARK7 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal tissues using PARK7 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using PARK7 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cance tissues using PARK7 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human human breast cancer tissues using PARK7 antibody with DAB staining. with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature . Incubate the tissue with the primary antibody diluted at a ratio of 1:200 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.| Product Name | Anti-PARK7 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PARK7 |
|---|---|
| Gene Synonyms | DJ1 DJ-1 GATD2 HEL-S-67p |
| Gene Full Name | Parkinsonism associated deglycase |
| Gene Summary | The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 20 kDa |
| Cellular Localization | Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus |
| SwissProt ID | Q99497 |
|---|
WB
Western blot analysis using PARK7 antibody against Hela(1), Thp-1(2), MCF-7(3), COS-7(4) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PARK7 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PARK7 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal tissues using PARK7 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using PARK7 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cance tissues using PARK7 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human human breast cancer tissues using PARK7 antibody with DAB staining. with heat-mediated antigen retrieval using sodium citrate buffer (pH 6.0) (OM750020) for 2 minutes. Wash the sections with ddH₂O and PBS (OM750003). Block the tissue with 10% non-immune goat serum(OM760028) at room temperature . Incubate the tissue with the primary antibody diluted at a ratio of 1:200 at 4°C overnight. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:200 and incubate for one hour. Tyramine labeled with 488 (Green,OM642679) was used as chromogenic agent, and DAPI(Blue,OM642679) was used for double dyeing, and the anti-fluorescence attenuation tablets were sealed.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
