WB
Western blot analysis using AMPK alpha antibody against K562(1), HepG2(2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using AMPK alpha antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human kidney tissues using AMPK alpha antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using AMPK alpha antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using AMPK alpha antibody with DAB staining.WB
(A) The expression of AdipoR1, AMPKα, p-AMPKα, PGC-1α in in subcutaneous adipose of pigs was detected by western blotting.| Product Name | Anti-AMPK alpha antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKAA1 |
|---|---|
| Gene Synonyms | AMPK AMPKa1 AMPK alpha 1 |
| Gene Full Name | protein kinase AMP-activated catalytic subunit alpha 1 |
| Gene Summary | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 62 kDa, 64 kDa |
| Cellular Localization | Cytoplasm; Nucleus |
WB
Western blot analysis using AMPK alpha antibody against K562(1), HepG2(2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using AMPK alpha antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human kidney tissues using AMPK alpha antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using AMPK alpha antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using AMPK alpha antibody with DAB staining.
WB
(A) The expression of AdipoR1, AMPKα, p-AMPKα, PGC-1α in in subcutaneous adipose of pigs was detected by western blotting.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
