WB
Western blot analysis using SPHK1 antibody against HEK293(1), L-02(2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using SPHK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using SPHK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer 2 tissues using SPHK1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using SPHK1 antibody with DAB staining.WB
Western blot analysis using SPHK1 antibody against Mouse liver(1),Rat intestine(2) tissue.| Product Name | Anti-SPHK1 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SPHK1 |
|---|---|
| Gene Synonyms | SPHK |
| Gene Full Name | sphingosine kinase 1 |
| Gene Summary | The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017] |
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Cell junction, Cell membrane, Coated pit, Cytoplasm, Endosome, Membrane, Nucleus, Synapse |
| SwissProt ID | Q9NYA1 |
|---|
WB
Western blot analysis using SPHK1 antibody against HEK293(1), L-02(2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using SPHK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using SPHK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer 2 tissues using SPHK1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissues using SPHK1 antibody with DAB staining.
WB
Western blot analysis using SPHK1 antibody against Mouse liver(1),Rat intestine(2) tissue.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
