ICC/IF
Immunofluorescence analysis of A549 cells using IDO antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.ICC/IF
IDO and CD4 immunofluorescence staining of wounds in mice on the 5th and 7th days post-transplantation. Note: Green represents IDO and red represents CD4. n = 5, the difference was statistically significant (***p < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)Previous| Product Name | Anti-IDO antibody-AF488 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | AF488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFFC |
| IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IDO1 |
|---|---|
| Gene Synonyms | IDO INDO IDO-1 |
| Gene Full Name | indoleamine 2,3-dioxygenase 1 |
| Gene Summary | This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011] |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P14902 |
|---|
ICC/IF
Immunofluorescence analysis of A549 cells using IDO antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.
ICC/IF
IDO and CD4 immunofluorescence staining of wounds in mice on the 5th and 7th days post-transplantation. Note: Green represents IDO and red represents CD4. n = 5, the difference was statistically significant (***p < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)Previous| Application Notes | IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
