WB
Western blot analysis using DNA Ligase IV antibody against SK-OV-3(1), Rb-1(2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using DNA Ligase IV antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using DNA Ligase IV antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human kidney tissues using DNA Ligase IV antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using DNA Ligase IV antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using DNA Ligase IV antibody with DAB staining.WB
Western blot analysis using DNA Ligase IV antibody against Rat serum(1) tissue.| Product Name | Anti-DNA Ligase IV antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LIG4 |
|---|---|
| Gene Synonyms | DNLI4 LIG4 LIG4S |
| Gene Full Name | DNA ligase 4 |
| Gene Summary | The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 104 kDa |
| Cellular Localization | Nucleus |
| SwissProt ID | P49917 |
|---|
WB
Western blot analysis using DNA Ligase IV antibody against SK-OV-3(1), Rb-1(2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using DNA Ligase IV antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using DNA Ligase IV antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human kidney tissues using DNA Ligase IV antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissues using DNA Ligase IV antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissues using DNA Ligase IV antibody with DAB staining.
WB
Western blot analysis using DNA Ligase IV antibody against Rat serum(1) tissue.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
