WB
Western blot analysis using CDC27 antibody against HEK293(1) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using CDC27 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using CDC27 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using CDC27 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using CDC27 antibody with DAB staining.| Product Name | Anti-CDC27 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDC27 |
|---|---|
| Gene Synonyms | APC3 HNUC NUC2 H-NUC ANAPC3 CDC27Hs D0S1430E D17S978E |
| Gene Full Name | cell division cycle 27 |
| Gene Summary | The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. |
| Molecular Weight(MW) | 92 kDa |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus |
| SwissProt ID | P30260 |
|---|
WB
Western blot analysis using CDC27 antibody against HEK293(1) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using CDC27 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using CDC27 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using CDC27 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using CDC27 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
