WB
Western blot analysis using RAG2 antibody against NIH3T3(1) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using RAG2 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RAG2 antibody with DAB staining.WB
Western blot analysis using RAG2 antibody against Mouse serum tissue(1),Mouse testes tissue(2).| Product Name | Anti-RAG2 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAG2 |
|---|---|
| Gene Synonyms | RAG-2 |
| Gene Full Name | recombination activating 2 |
| Gene Summary | This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 59KD |
| Cellular Localization | Nucleus |
| SwissProt ID | P55895 |
|---|
WB
Western blot analysis using RAG2 antibody against NIH3T3(1) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using RAG2 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RAG2 antibody with DAB staining.
WB
Western blot analysis using RAG2 antibody against Mouse serum tissue(1),Mouse testes tissue(2).| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
