IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using ERK3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ERK3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer 2 tissues using ERK3 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using ERK3 antibody with DAB staining.WB
Western blot analysis using ERK3 antibody against Mouse testes tissue(1),Mouse liver tissue(2),Rat kidney tissue(3),Rat liver tissue(4). gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60| Product Name | Anti-ERK3 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAPK6 |
|---|---|
| Gene Synonyms | ERK3 PRKM6 p97MAPK HsT17250 |
| Gene Full Name | mitogen-activated protein kinase 6 |
| Gene Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 83KD(observed:100kDa) |
| Cellular Localization | Cytoplasm, Nucleus |
| SwissProt ID | Q16659 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissues using ERK3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using ERK3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer 2 tissues using ERK3 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissues using ERK3 antibody with DAB staining.
WB
Western blot analysis using ERK3 antibody against Mouse testes tissue(1),Mouse liver tissue(2),Rat kidney tissue(3),Rat liver tissue(4). gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000-1:2000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
