IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RAF1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancerr tissues using RAF1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using RAF1 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using RAF1 antibody with DAB staining.| Product Name | Anti-RAF1 antibody-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | RAF1 |
|---|---|
| Gene Synonyms | NS5 CRAF Raf-1 c-Raf CMD1NN |
| Gene Full Name | Raf-1 proto-oncogene, serine/threonine kinase |
| Gene Summary | This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 73KD |
| Cellular Localization | Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus |
| SwissProt ID | P04049 |
|---|
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using RAF1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancerr tissues using RAF1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using RAF1 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using RAF1 antibody with DAB staining.| Application Notes | IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
