WB
Western blot analysis using PSMB8 antibody against HEK293(1), Hela(2), NiH3T3(3) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using PSMB8 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using PSMB8 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using PSMB8 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PSMB8 antibody with DAB staining.IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using PSMB8 antibody with DAB staining.| Product Name | Anti-PSMB8 antibody-poly-HRP |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Conjugation | poly-HRP |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:200-1:2000 IHC:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PSMB8 |
|---|---|
| Gene Synonyms | PSB8 JMP ALDD LMP7 NKJO D6S216 PRAAS1 PSMB5i RING10 D6S216E |
| Gene Full Name | proteasome 20S subunit beta 8 |
| Gene Summary | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 30kD(Observed:45 kD） |
| Cellular Localization | Cytoplasm, Nucleus, Proteasome |
| SwissProt ID | P28062 |
|---|
WB
Western blot analysis using PSMB8 antibody against HEK293(1), Hela(2), NiH3T3(3) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissues using PSMB8 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human colon cancer tissues using PSMB8 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissues using PSMB8 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissues using PSMB8 antibody with DAB staining.
IHC
Immunohistochemical analysis of paraffin-embedded Human rectal cancer tissues using PSMB8 antibody with DAB staining.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
